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(A–C) Positive and negative controls of tonsillar tissue. (A) LLT1 expression showing membranous/cytoplasmic staining in lymphoid cells within tonsillar follicles. (B) <t>CD161</t> expression highlighting scattered lymphocytes in the interfollicular areas. (C) Negative control by omission of the primary antibody showed complete absence of staining (IHC stain, 20x).
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(A–C) Positive and negative controls of tonsillar tissue. (A) LLT1 expression showing membranous/cytoplasmic staining in lymphoid cells within tonsillar follicles. (B) <t>CD161</t> expression highlighting scattered lymphocytes in the interfollicular areas. (C) Negative control by omission of the primary antibody showed complete absence of staining (IHC stain, 20x).
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(A–C) Positive and negative controls of tonsillar tissue. (A) LLT1 expression showing membranous/cytoplasmic staining in lymphoid cells within tonsillar follicles. (B) <t>CD161</t> expression highlighting scattered lymphocytes in the interfollicular areas. (C) Negative control by omission of the primary antibody showed complete absence of staining (IHC stain, 20x).
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(A–C) Positive and negative controls of tonsillar tissue. (A) LLT1 expression showing membranous/cytoplasmic staining in lymphoid cells within tonsillar follicles. (B) CD161 expression highlighting scattered lymphocytes in the interfollicular areas. (C) Negative control by omission of the primary antibody showed complete absence of staining (IHC stain, 20x).

Journal: Frontiers in Medicine

Article Title: Reed-Sternberg cells express CD161 and lectin-like transcript 1 in Hodgkin lymphoma

doi: 10.3389/fmed.2026.1754727

Figure Lengend Snippet: (A–C) Positive and negative controls of tonsillar tissue. (A) LLT1 expression showing membranous/cytoplasmic staining in lymphoid cells within tonsillar follicles. (B) CD161 expression highlighting scattered lymphocytes in the interfollicular areas. (C) Negative control by omission of the primary antibody showed complete absence of staining (IHC stain, 20x).

Article Snippet: The sections were incubated with primary antibodies specific to CD161 (anti-KLRB1/CD161 mAb clone 3D8F5, Proteintech Catalog No. 67537-1-Ig, RRID: AB_2882756 , 1:1,000), LLT1 (anti-CLEC2D mAb clone 4C7, Abnova, Catalog No. H00029121-M01, RRID: AB_463941 , 1:50), CD8 (anti-CD8 mAb clone 4B11, MyBioSource Catalog No. MBS215242, RRID: AB_10887243 , 1:50), and EBV (anti-EBV/LMP-1 mAb clone S20-D, MyBioSource Catalog No. MBS684063, 1:100).

Techniques: Expressing, Staining, Negative Control

(A–F) . CD161 expression patterns in Reed–Sternberg RS cells by immunohistochemistry (40x and 100x inset). (A) No detectable CD161 expression in RS cells, background immune cells are positive. (B) Low-level cytoplasmic only expression. (C) High-level cytoplasmic only expression. (D) High-level membranous-only expression. (E) Low-level dual cytoplasmic and membranous expression. (F) High-level dual cytoplasmic and membranous expression. The total expression level was calculated by multiplying the stain intensity score by the percentage of positive RS cells. Based on the median score, patients were classified into low expression (≤90; median), and high expression (>90; median) groups.

Journal: Frontiers in Medicine

Article Title: Reed-Sternberg cells express CD161 and lectin-like transcript 1 in Hodgkin lymphoma

doi: 10.3389/fmed.2026.1754727

Figure Lengend Snippet: (A–F) . CD161 expression patterns in Reed–Sternberg RS cells by immunohistochemistry (40x and 100x inset). (A) No detectable CD161 expression in RS cells, background immune cells are positive. (B) Low-level cytoplasmic only expression. (C) High-level cytoplasmic only expression. (D) High-level membranous-only expression. (E) Low-level dual cytoplasmic and membranous expression. (F) High-level dual cytoplasmic and membranous expression. The total expression level was calculated by multiplying the stain intensity score by the percentage of positive RS cells. Based on the median score, patients were classified into low expression (≤90; median), and high expression (>90; median) groups.

Article Snippet: The sections were incubated with primary antibodies specific to CD161 (anti-KLRB1/CD161 mAb clone 3D8F5, Proteintech Catalog No. 67537-1-Ig, RRID: AB_2882756 , 1:1,000), LLT1 (anti-CLEC2D mAb clone 4C7, Abnova, Catalog No. H00029121-M01, RRID: AB_463941 , 1:50), CD8 (anti-CD8 mAb clone 4B11, MyBioSource Catalog No. MBS215242, RRID: AB_10887243 , 1:50), and EBV (anti-EBV/LMP-1 mAb clone S20-D, MyBioSource Catalog No. MBS684063, 1:100).

Techniques: Expressing, Immunohistochemistry, Staining

Overall survival (OS) according to CD161 expression in RS cells. Kaplan-Meier curves OS stratified by LLT1 expression in RS cells based on immunohistochemistry score median. Patients were grouped into low LLT1 expression (≤90) and high LLT1 expression (>90). Tick marks indicate censored (alive/lost) cases. No statistically significant difference in overall survival was observed among groups with different CD161 expression levels in RS cells ( p -value = 0.277).

Journal: Frontiers in Medicine

Article Title: Reed-Sternberg cells express CD161 and lectin-like transcript 1 in Hodgkin lymphoma

doi: 10.3389/fmed.2026.1754727

Figure Lengend Snippet: Overall survival (OS) according to CD161 expression in RS cells. Kaplan-Meier curves OS stratified by LLT1 expression in RS cells based on immunohistochemistry score median. Patients were grouped into low LLT1 expression (≤90) and high LLT1 expression (>90). Tick marks indicate censored (alive/lost) cases. No statistically significant difference in overall survival was observed among groups with different CD161 expression levels in RS cells ( p -value = 0.277).

Article Snippet: The sections were incubated with primary antibodies specific to CD161 (anti-KLRB1/CD161 mAb clone 3D8F5, Proteintech Catalog No. 67537-1-Ig, RRID: AB_2882756 , 1:1,000), LLT1 (anti-CLEC2D mAb clone 4C7, Abnova, Catalog No. H00029121-M01, RRID: AB_463941 , 1:50), CD8 (anti-CD8 mAb clone 4B11, MyBioSource Catalog No. MBS215242, RRID: AB_10887243 , 1:50), and EBV (anti-EBV/LMP-1 mAb clone S20-D, MyBioSource Catalog No. MBS684063, 1:100).

Techniques: Expressing, Immunohistochemistry

Progression-free survival (PFS) according to CD161 expression in RS cells. Kaplan–Meier curves showing progression-free survival (PFS) stratified by LLT1 expression in Reed–Sternberg (RS) cells based on the median immunohistochemistry (IHC) score. Patients were categorized into low LLT1 expression (≤90) and high LLT1 expression (>90). Tick marks indicate censored (alive/lost) cases. No statistically significant difference in progression-free survival between patients with low and high LLT1 expression on RS cells ( p = 0.734).

Journal: Frontiers in Medicine

Article Title: Reed-Sternberg cells express CD161 and lectin-like transcript 1 in Hodgkin lymphoma

doi: 10.3389/fmed.2026.1754727

Figure Lengend Snippet: Progression-free survival (PFS) according to CD161 expression in RS cells. Kaplan–Meier curves showing progression-free survival (PFS) stratified by LLT1 expression in Reed–Sternberg (RS) cells based on the median immunohistochemistry (IHC) score. Patients were categorized into low LLT1 expression (≤90) and high LLT1 expression (>90). Tick marks indicate censored (alive/lost) cases. No statistically significant difference in progression-free survival between patients with low and high LLT1 expression on RS cells ( p = 0.734).

Article Snippet: The sections were incubated with primary antibodies specific to CD161 (anti-KLRB1/CD161 mAb clone 3D8F5, Proteintech Catalog No. 67537-1-Ig, RRID: AB_2882756 , 1:1,000), LLT1 (anti-CLEC2D mAb clone 4C7, Abnova, Catalog No. H00029121-M01, RRID: AB_463941 , 1:50), CD8 (anti-CD8 mAb clone 4B11, MyBioSource Catalog No. MBS215242, RRID: AB_10887243 , 1:50), and EBV (anti-EBV/LMP-1 mAb clone S20-D, MyBioSource Catalog No. MBS684063, 1:100).

Techniques: Expressing, Immunohistochemistry